Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

Blog Article

RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions.NMR Upholstered Storage Bench spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution.Still, RNA analysis by NMR suffers from spectral overlap and line broadening, both of which worsen for larger RNAs.Incorporation of stable isotope labels into RNA has provided several solutions Kids Watch to these challenges.In this review, we summarize the benefits and limitations of various methods used to obtain isotope-labeled RNA building blocks and how they are used to prepare isotope-labeled RNA for NMR structure and dynamics studies.